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herringbone structured pdms microchannel  (Dow Corning)

 
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    Structured Review

    Dow Corning herringbone structured pdms microchannel
    Herringbone Structured Pdms Microchannel, supplied by Dow Corning, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microchannel/herringbone+microchannel+pdms+structured/10__1016_slash_j__device__2026__101153-275-1-22
    Average 86 stars, based on 1 article reviews
    herringbone structured pdms microchannel - by Bioz Stars, 2026-10
    86/100 stars

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    Article Title: Particle separation with microfluidic device using standing surface acoustic waves
    Article Snippet: Microfluidics is a field that involves the manipulation of fluids at the micron-scale, enabling precise control and analysis of small volumes of samples and reagents [1].. Due to its advantages of high efficiency, low consumption and easy integration, it has developed rapidly in the fields of biology, chemistry and medicine [2-4].. Additionally, microfluidic device has application in analytical analysis, lab-on-a-chip technology and cell sorting and separation [5, 6].

    Article Title: Cell detachment model for an antibody-based microfluidic cancer screening system.
    Article Snippet: We consider cells bound to the floor of a microfluidic channel and present a model of their flow-induced detachment.. We approximate hydrodynamic force and cell elastic response using static finite-element simulation of a single cell.. Detachment is assumed to occur when hydrodynamic and adhesive forces are roughly equal.

    Article Title: Hydrogen generation and utilization in a two-phase flow membraneless microfluidic electrolyzer-fuel cell tandem operation for micropower application
    Article Snippet: Advances in device miniaturization, ranging from sensors to transmitters, are driving the need for micro-scaled personalized on-demand power sources.. Herein, the tandem operation of a membraneless, two-phase flow microfluidic electrolysis cell and fuel cell (μEC-μFC) is demonstrated, which is a step towards developing an independent micropower source.. This device consists of a membraneless double Y-shaped microchannel that encompasses microelectrodes for the microfluidic electrolysis cell (μEC) and microfluidic fuel cell (μFC).

    Article Title: Continuous generation of hydrogel beads and encapsulation of biological materials using a microfluidic droplet-merging channel
    Article Snippet: In this paper, we describe a method for encapsulation of biomaterials in hydrogel beads using a microfluidic droplet-merging channel.. We devised a double T-junction in a microfluidic channel for alternate injection of aqueous fluids inside a droplet unit carried within immiscible oil.. With this device, hydrogel beads with diameter\100 lm are produced, and various solutions containing cells, proteins and reagents for gelation could merge with the gel droplets with high efficiency in the broad range of flow rates.

    Control:

    Article Title: Development and characterization of hollow microprobe array as a potential tool for versatile and massively parallel manipulation of single cells.
    Article Snippet: Parallel manipulation of single cells is important for reconstructing in vivo cellular microenvironments and studying cell functions.. To manipulate single cells and reconstruct their environments, development of a versatile manipulation tool is necessary.. In this study, we developed an array of hollow probes using microelectromechanical systems fabrication technology and demonstrated the manipulation of single cells.

    Microscopy:

    Article Title: Influence of the internal structure of straight microchannels on inertial transport behavior of particles
    Article Snippet: .. The specific steps of the experimental operation are as follows. (1) Place the microchannel fabricated from PDMS (Dow Corning, USA) through photolithography under the microscope and secure it. ..



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    Design and physical characterization of the microchannel device. (A) Photographic image of two microfluidic devices. Each device features one inlet and one outlet connector to a cell culture chamber (filled with methylene blue dye for better visualisation). The microchannel structures were made from polydimethylsiloxane (PDMS) and bonded to a cyclo-olefin copolymer (COC) microscope slide. Scale bar = 10 mm. (B) Top view drawing of the microchannel device with corresponding key dimensions represented in millimetres. The area of the culture chamber is approximately 0.39 cm 2 , similar to the surface area of a single well of a 96-well plate (0.32 cm 2 ). (C) Characterisation of microchannel depth by optical profilometry. The micro-machined height of the polycarbonate (PC) mould and the corresponding depths of the PDMS channels were compared to verify the accuracy of the PDMS casting. The channel depths expected by the design are indicated by the dashed lines. All data points are presented as mean ±1 standard deviation of technical triplicates. Significance was assessed using un-paired T-test (p > 0.05).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays

    doi: 10.3389/fbioe.2026.1720882

    Figure Lengend Snippet: Design and physical characterization of the microchannel device. (A) Photographic image of two microfluidic devices. Each device features one inlet and one outlet connector to a cell culture chamber (filled with methylene blue dye for better visualisation). The microchannel structures were made from polydimethylsiloxane (PDMS) and bonded to a cyclo-olefin copolymer (COC) microscope slide. Scale bar = 10 mm. (B) Top view drawing of the microchannel device with corresponding key dimensions represented in millimetres. The area of the culture chamber is approximately 0.39 cm 2 , similar to the surface area of a single well of a 96-well plate (0.32 cm 2 ). (C) Characterisation of microchannel depth by optical profilometry. The micro-machined height of the polycarbonate (PC) mould and the corresponding depths of the PDMS channels were compared to verify the accuracy of the PDMS casting. The channel depths expected by the design are indicated by the dashed lines. All data points are presented as mean ±1 standard deviation of technical triplicates. Significance was assessed using un-paired T-test (p > 0.05).

    Article Snippet: Microchannel moulds were designed in Fusion v2.0.20981 (Autodesk, United States), manufactured using CNC milling from 3 mm thick polycarbonate (PC) sheets.

    Techniques: Cell Culture, Microscopy, Standard Deviation

    Comparison of transduction efficiency (TE) obtained by maintaining constant either the (A) MOI of 2 or the (B) LVV concentration (TU mL -1 ) of 6.3 × 10 5 TU mL -1 . This was evaluated across 3 microchannels with different culture fluid overlay with a 24h incubation between the LVV particles and target cells. (C) TE evaluated across a range of MOI values: 0.5, 1, 2 and 4. All experiments were performed in parallel in a 96-well plate and a set of microchannels representing various channel depths. (D) Transduction efficiency comparing a 96-well plate well with an ∼1.5 mm culture overlay and a 1.5 mm microchannel. Data points are presented as mean ±1 standard deviation (n = 2, 3). Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays

    doi: 10.3389/fbioe.2026.1720882

    Figure Lengend Snippet: Comparison of transduction efficiency (TE) obtained by maintaining constant either the (A) MOI of 2 or the (B) LVV concentration (TU mL -1 ) of 6.3 × 10 5 TU mL -1 . This was evaluated across 3 microchannels with different culture fluid overlay with a 24h incubation between the LVV particles and target cells. (C) TE evaluated across a range of MOI values: 0.5, 1, 2 and 4. All experiments were performed in parallel in a 96-well plate and a set of microchannels representing various channel depths. (D) Transduction efficiency comparing a 96-well plate well with an ∼1.5 mm culture overlay and a 1.5 mm microchannel. Data points are presented as mean ±1 standard deviation (n = 2, 3). Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Article Snippet: Microchannel moulds were designed in Fusion v2.0.20981 (Autodesk, United States), manufactured using CNC milling from 3 mm thick polycarbonate (PC) sheets.

    Techniques: Comparison, Transduction, Concentration Assay, Incubation, Standard Deviation

    Assessment of linearity, reproducibility and incubation time of the microchannel assays. (A) Linearity evaluated using 4 MOI values, using the following channel depth: 1.5 mm (plate) and 1, 0.6 and 0.2 mm (microchannels). (1.5 mm: R 2 = 0.9593; 1.0 mm: R 2 = 0.8721; 0.6 mm: R 2 = 0.9270; 0.2 mm: R 2 = 0.9798). (B) Assay reproducibility evaluated across three experimental occasions for each channel depth (mm). Each experiment was performed in technical triplicates, i. e., for every channel depth three devices were evaluated in parallel. (CV: 1.0 mm: 11%; 0.6 mm: 3.4%; 0.2 mm: 3.9%). (C) Heatmap outlining the extent of transduction efficiency obtained across several incubation times between the LVV material and target cells. This is performed at a constant MOI of 1. All data is representative of the mean from triplicate measurements ±1 standard deviation. Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays

    doi: 10.3389/fbioe.2026.1720882

    Figure Lengend Snippet: Assessment of linearity, reproducibility and incubation time of the microchannel assays. (A) Linearity evaluated using 4 MOI values, using the following channel depth: 1.5 mm (plate) and 1, 0.6 and 0.2 mm (microchannels). (1.5 mm: R 2 = 0.9593; 1.0 mm: R 2 = 0.8721; 0.6 mm: R 2 = 0.9270; 0.2 mm: R 2 = 0.9798). (B) Assay reproducibility evaluated across three experimental occasions for each channel depth (mm). Each experiment was performed in technical triplicates, i. e., for every channel depth three devices were evaluated in parallel. (CV: 1.0 mm: 11%; 0.6 mm: 3.4%; 0.2 mm: 3.9%). (C) Heatmap outlining the extent of transduction efficiency obtained across several incubation times between the LVV material and target cells. This is performed at a constant MOI of 1. All data is representative of the mean from triplicate measurements ±1 standard deviation. Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Article Snippet: Microchannel moulds were designed in Fusion v2.0.20981 (Autodesk, United States), manufactured using CNC milling from 3 mm thick polycarbonate (PC) sheets.

    Techniques: Incubation, Transduction, Standard Deviation

    Identifying the impact of microchannel transductions on assay sensitivity compared with 96-well plates. (A) Quantification of vector genome copies per cell, measured by qPCR. (B) The fraction of GFP-positive cells determined by flow cytometry. All values are presented as mean of n = 3 with error bars indicating ±1 standard deviation of the mean. Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays

    doi: 10.3389/fbioe.2026.1720882

    Figure Lengend Snippet: Identifying the impact of microchannel transductions on assay sensitivity compared with 96-well plates. (A) Quantification of vector genome copies per cell, measured by qPCR. (B) The fraction of GFP-positive cells determined by flow cytometry. All values are presented as mean of n = 3 with error bars indicating ±1 standard deviation of the mean. Significance was assessed using one-way ANOVA with Dunnett’s post hoc analysis (ns: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001).

    Article Snippet: Microchannel moulds were designed in Fusion v2.0.20981 (Autodesk, United States), manufactured using CNC milling from 3 mm thick polycarbonate (PC) sheets.

    Techniques: Plasmid Preparation, Flow Cytometry, Standard Deviation